Coding

Part:BBa_K2928002:Design

Designed by: Genta Firth   Group: iGEM19_ASIJ_Tokyo   (2019-10-10)


Modified 0.19 Wheat Albumin sequence (Gly to Glu), an α-Amylase inhibitor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We spent many hours finding the strongest repelling amino acids between α-amylase and 0.19 to improve the attraction between them.


Source

The original 0.19 Wheat albumin came from the PDB and used Pymol and INTAA Server to model and map the amino acid attractions, to make the best amino acid substitution.

References